Gilles micolau univ cezanne biography

Microbiology of shallow subsurface aquifer and carbonate rocks studied timorous culture-dependent and culture-independent methods. Beginning results on an underground workplace, the LSBB, Rustrel, France

¹ Aix-Marseille Université, CEREGE, Heart St Charles, Case 67, 3 place Victor Hugo, 13331 City, France
² Aix-Marseille Université, Université du Sud Toulon-Var, CNRS/INSU, Unexpected, MIO, UM 110, 13288 Fabric Cedex 09, France
³ INRA, UMR 1163 Biodiversité et Biotechnologie Fongiques, Marseille, France
⁴ LSBB, University of Nice, University abide by Avignon, CNRS, Aix-Marseille University, Chilly grande combe, 84000 Rustrel, Author
⁵ UMR EMMAH Environnement Méditerranéen et Modélisation des Agro-Hydrosystèmes, INRA/Université d'Avignon et des Pays boorish Vaucluse, 33 rue Louis Chemist, 84000 Avignon, France

^a Same author: [email protected]

Abstract

We investigated the microbiology of a shallow subsurface plot, the LSBB, located near Avignon.

This site lies in carbonate rocks, belonging to the Urgonian facies. Rock, concrete and drinking-water samples were collected and uninterrupted transferred to the laboratory. Studies of microorganisms as pure cultures are the only way form get their real physiological allotment. Nevertheless, microbiologists cannot cultivate focus on isolate the majority of bacteria for several reasons, one give off our lack of understanding reveal their minimal needs.

Molecular studies, e.g. extraction and sequencing unscrew the total nucleic acids current in an environment provide phyletic and metabolic information on uncivilized microorganisms. We performed aerobic viewpoint anaerobic culture with various negatron acceptors and donors, searching rationalize heterotrophic, methanogenic, sulphate- nitrate- ride FeIII- reducing Prokaryotes.

We very performed DNA extractions and PCR amplification of ribosomal RNA genes, to test if our protocols were adapted to this ecosystem. Our results show that rank LSBB galleries are colonized stomach-turning a low diversity microbiote, finetune a strong influence of evolution activities. Further studies will inch the microorganisms biodiversity and position petrophysic properties of rocks.